Description

Wolverine Blend (BPC-157 & TB-500 Peptide Blend)

For Research & Laboratory Use Only

Overview

The Wolverine Blend combines two synthetic research peptides — BPC-157 and TB-500 — each widely studied in experimental models of tissue repair, cellular regeneration, and inflammation control.

BPC-157 (Pentadecapeptide BPC-157) is a 15–amino-acid peptide derived from a larger protective protein found in gastric tissue. Research suggests that BPC-157 may interact with cellular communication pathways involved in angiogenesis, inflammation modulation, and tissue repair. Experimental findings also propose potential influence on collagen production, fibroblast activity, nerve regeneration, and vascular stability.(1)

TB-500 (Synthetic Thymosin Beta-4) is a laboratory-created analog of the naturally occurring thymosin beta-4 (Tβ4) peptide, encoded by the TMSB4X gene. TB-500 research suggests potential roles in cell migration, wound remodeling, angiogenesis, and tissue renewal. Investigations also highlight possible effects on inflammation-related signaling and neurological recovery pathways.(2)

Both peptides are synthetic polypeptides:

• BPC-157: 15 amino acids

• TB-500: 43 amino acids(3,4)

When studied together, their mechanistic overlap in cellular repair, angiogenesis, and inflammation control suggests the possibility of complementary or synergistic responses in experimental settings.

Mechanistic Overview

TB-500 (Tβ4 Analogue) – Cell Motility, Actin Regulation, & Inflammation Pathways

Based on thymosin beta-4 research, TB-500 appears to influence cell movement and differentiation by interacting with the actin cytoskeleton. A highly conserved amino acid sequence — LKKTETQ (positions 17–23) — is believed to bind G-actin, potentially enhancing cell migration and structural remodeling.(5)

Studies also propose possible upregulation of microRNA-146a, a regulatory molecule associated with inflammation control. Increased miR-146a levels have been linked to suppressed IRAK1 and TRAF6 activity, two signaling nodes connected to inflammatory cytokine responses.(6)

These observations point to a potential dual role in cellular repair and inflammation modulation.

BPC-157 – Angiogenic Activity, NO Signaling, and Tissue Remodeling

BPC-157 has been investigated for its potential involvement in nitric oxide pathways, endothelial protection, and angiogenesis. Experimental data suggest it may influence:

• Early growth response gene-1 (EGR1) expression

• Collagen organization

• Extracellular matrix formation

• Fibroblast migration

• Nerve-related growth pathways(7,8)

Findings also indicate potential support for tendon outgrowth, increased fibroblast mobility, and enhanced cellular survival via F-actin activation.

Combined Experimental Rationale

Because both TB-500 and BPC-157 have been independently studied for similar cellular processes — wound repair, collagen formation, angiogenic support, and modulation of inflammatory signaling — combining them may provide overlapping or amplified potential responses in research settings.

Chemical Makeup

Molecular Formula

• BPC-157: C₆₂H₉₈N₁₆O₂₂

• TB-500: C₂₁₂H₃₅₀N₅₆O₇₈S

Molecular Weight

• BPC-157: 1419.5 g/mol

• TB-500: 4963 g/mol

Other Names

• BPC-157: Body Protection Compound-157

• TB-500: Thymosin Beta-4

Research and Experimental Findings

1. Tissue Repair and Wound Models

TB-500 (Tβ4) Studies:
A landmark murine wound model (1999) found that animals receiving TB-500 demonstrated a 41% increase in re-epithelialization at day 4 and 11% greater wound contraction by day 7 compared to saline controls.(9)

A separate clinical trial in patients with pressure ulcers found progressive signs of repair over an 84-day period in peptide-treated groups.(10)

BPC-157 Studies:
Murine models with acute and chronic wounds showed increased collagen formation and angiogenesis when exposed to BPC-157 compared to placebo.(11)

2. Ligament & Tendon Models

TB-500:
When the medial collateral ligament (MCL) was surgically transected, TB-500–treated animals displayed more uniform, thicker collagen fibers and improved mechanical tissue properties after four weeks.(12)

BPC-157:
Studies suggest BPC-157 may support tendon explant growth, fibroblast survival, and migration, potentially mediated through F-actin formation and FAK-paxillin pathway activation.(8)

3. Muscle Repair

BPC-157 has been studied in corticosteroid-damaged skeletal muscle, where treated animals showed near-complete restoration of muscle structure and function within 14 days, compared to no meaningful improvement in placebo groups.(13)

TB-500 research suggests potential protective and regenerative effects in cardiac muscle models, including:

• Improved cardiomyocyte survival

• Enhanced angiogenesis

• Increased ILK and Akt signaling(14,15)

These observations reinforce interest in TB-500 as a subject for experimental study in cardiac and skeletal muscle cell models.

Research-Use Only Disclaimer

Wolverine Blend (BPC-157 & TB-500) from OptiBuild Peptides is supplied strictly for laboratory, scientific, and in-vitro research purposes only.
Not for human use, medical applications, diagnostic procedures, or veterinary administration.
All purchasers must follow applicable regulations and our Terms & Conditions.

References

1. Seiwerth, S., Milavic, M., Vukojevic, J., et al. Stable Gastric Pentadecapeptide BPC 157 and Wound Healing. Frontiers in Pharmacology, 2021. https://doi.org/10.3389/fphar.2021.627533

2. Maar, K., Hetenyi, R., Maar, S., et al. Utilizing Developmentally Essential Secreted Peptides Such as Thymosin Beta-4… Cells, 2021. https://doi.org/10.3390/cells10061343

3. PubChem Compound Summary (CID 132558700) – TB-500.

4. PubChem Compound Summary (CID 9941957) – BPC-157.

5. Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature, 2008. https://pubmed.ncbi.nlm.nih.gov/18480812/

6. Santra M, Zhang ZG, Yang J, et al. Tβ4 up-regulation of microRNA-146a… J Biol Chem, 2014.

7. Sikiric P., et al. Brain-Gut Axis and Pentadecapeptide BPC-157. Curr Neuropharmacol, 2016.

8. Chang C-H., et al. BPC-157 and tendon healing. J Appl Physiol, 2011.

9. Malinda KM, et al. Thymosin β4 Accelerates Wound Healing. J Invest Dermatol, 1999.

10. ClinicalTrials.gov – Study of Thymosin Beta 4 in Patients With Pressure Ulcers.

11. Seiwerth S., et al. BPC-157’s effect on healing. J Physiol Paris, 1997.

12. Xu B., et al. Tβ4 enhances healing of medial collateral ligament injury. Regul Pept, 2013.

13. Pevec D., et al. Impact of BPC-157 on muscle healing impaired by systemic corticosteroids. Med Sci Monit, 2010.

14. Srivastava D., et al. Cardiac repair with thymosin β4 and reprogramming factors. Ann NY Acad Sci, 2012.

15. Bock-Marquette I., et al. Thymosin β4 activates ILK and promotes cardiac repair. Nature, 2004.